International Journal of Biomedicine. 2021;11(1):50-52.
Originally published March 5, 2021
The purpose of the this research was to obtain and study the properties of copper-pectic gel particles (CuPGPs) obtained from aqueous solutions of apple pectin (AP) in the concentration range of 1%-5% in the presence of Cu2+ ions.
Methods and Results: We used commercial AP AU701 (Herbstreith & Fox KG, Germany). CuPGPs were obtained from aqueous solutions of AP (1%, 3%, 5%) in the presence of Cu2+ ions (1%-10%) by the method of ionotropic gelation, The diameter and density of the CuPGPs were determined.
Dry CuPGPs formed from 5% AP with all tested concentrations of copper ions have the largest diameter (0.96-1.15mm), and gel particles formed on the basis of 1% AP have the smallest diameter (0.42-0.74mm). CuPGPs formed from 5% AP have the highest density (1.43-1.65 mg/mm3), and CuPGPs formed on the basis of 1% AP have the lowest density (0.65-0.92 mg/mm3). Gel particles obtained from 1% AP swelled in simulated gastric fluid (SGF) by 161% and then completely degraded immediately upon entering in simulated intestinal fluid (SIF). CuPGPs obtained from 3% AP swelled by 166% in simulated gastric fluid (SGF) and 148% in SIF, and completely degraded in SIF after 2.5 hours of incubation in it. Gel particles obtained from 5% AP in the presence of 10% Cu2+ swelled most strongly – by 173% in SGF and by 208% in SIF. And then, they degraded after 8 hours of incubation in simulated colonic fluid (SCF).
1. Braccini I, Pérez S. Molecular basis of Ca2+-induced gelation in alginates and pectins: the egg-box model revisited. Biomacromolecules. 2001;2(4):1089-96. doi: 10.1021/bm010008g.
2. Volkova MV, Khasanshina ZR, Popov SV, Markov PA. [Gel granules from pectin callus Lupinus angustifolius prevent the premature release of mesalazine]. Biotechnology. 2020;36(1):53-60. doi: 10.21519/0234-2758-2020-36-1-53-60. [Article in Russian].
3. Seslija S, Veljovic D, Krusic MK, Stevanovic J, Velickovic S, Popovic I. Cross-linking of highly methoxylated pectin with copper: the specific anion influence. New J Chem. 2016;40:1618-1625. doi: 10.1039/C5NJ0332A
4. Williams RJP. Missing information in bio-inorganic chemistry. Coord Chem Rev. 1987;79(3):175-193. doi: 10.1016 / 0010-8545 (87) 80002-4
5.Shubakov AA, Mikhailova EA. Production, properties and transit of copper-pectic gel particles through an artificial gastroenteric environment. International Journal of Biomedicine. 2020;10(4):421-423.doi: 10.21103/Article10(4)_OA18
6. Sriamornsak P. Effect of calcium concentration, hardening agent and drying condition on release characteristics of oral proteins from calcium pectinate gel beads. Eur J Pharm Sci. 1999 Jul;8(3):221-7. doi: 10.1016/s0928-0987(99)00010-x.
7. Sriamornsak P, Nunthanid J. Calcium pectinate gel beads for controlled release drug delivery: I. Preparation and in vitro release studies. Int J Pharm. 1998;160: 207-212. doi: PII S0378-5173 (97) 00310-4
8. Gebara C, Chaves KS, Ribeiro MCE, Souza FN, Grosso CRF, Gigante ML. Viability of Lactobacillus acidophilus La5 in pectin-whey protein microparticles during exposure to simulated gastrointestinal conditions. Food Res Int. 2013;51:872-878. doi: 10.1016/j.foodres.2013.02.008
9. Shubakov AA, Mikhailova EA, Prosheva VI, Belyy VA. Swelling and degradation of calcium-pectic gel particles made of pectins of Silene vulgaris and Lemna minor callus cultures at different concentrations of pectinase in an artificial colon environment. International Journal of Biomedicine. 2018; 8(1):60-64.doi: 10.21103/Article8(1)_OA10
10. Oliveira GF, Ferrari PC, Carvalho LQ, Evangelista RC. Chitosan-pectin multiparticulate systems associated with enteric polymers for colonic drug delivery. Carbohydr Polym. 2010;82(3):1004-1009. doi: 10.1016/j.carbpol.2010.06.041
11. Mikhailova EA, Melekhin AK, Belyy VA, Shubakov AA. Stability of hyaluronan-pectic gel particles in the conditions of the artificial gastrointestinal environment. International Journal of Biomedicine. 2017;7(4):310-314. doi: 10.21103/Article7(4) _OA8
12. Sriamornsak P, Kennedy RA. Swelling and diffusion studies of calcium polysaccharide gels intended for film coating. Int J Pharm. 2008 Jun 24;358(1-2):205-13. doi: 10.1016/j.ijpharm.2008.03.009.
13. Gȕnter EA, Popeyko OV, Istomina EI. [Preparation and properties of hydrogel matrices based on pectins from callus cultures]. Biotechnology. 2020;36(3):63-72. doi: 10/21519/0234-2758-2020-36-3-63-72. [Article in Russian].
14. Rodrigues JR, Lagoa R. Copper ions binding in Cu-alginate gelation. J Carbohydr Chem. 2006;25:219-232.doi: 0.1080 / 07328300600732956
Received January 31, 2021.
Accepted February 25, 2021.
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