Tashkent State Dental Institute; Tashkent, Uzbekistan
*Corresponding author: Umida A. Shukurova, PhD. Tashkent State Dental Institute; Tashkent, Uzbekistan. E-mail: firstname.lastname@example.org
Published: March 16, 2016. DOI: 10.21103/Article6(1)_OA16
The aim of the study was to assess the content of short chain fatty acids (SCFAs) in feces of patients with different clinical forms of oral lichen planus (OLP).
Materials and Methods: The study included 139 patients with different clinical forms of OLP in the acute stage. The diagnosis of OLP was performed according to both clinical and histopathological criteria. Patients were distributed in four groups according to the clinical form of OLP. Group 1 included 36 patients with the reticular form of OLP; Group 2 included 34 patients with the exudative-hyperemic form of OLP; Group 3 included 27 patients with the erosive-ulcerative form of OLP; Group 4 included 42 patients with the bullous form of OLP. The four groups to be compared were randomized by sex and age. The control group consisted of 40 healthy, age-matched, randomly selected persons without clinical and instrumental signs of OLP and other diseases of the oral cavity. The concentration of SCFAs in feces was evaluated by gas-liquid chromatography. The profiles (specific concentration) of C2, C3, and C4 acids, the ratio of iso-acids to straight-chain acids (iso-Cn/Cn) and iso-C5/C5 were also calculated.
Results: Three types of SCFA changes reflecting the activity of certain groups of microorganisms were found. For all types of the SCFA disorders, we found a marked increase in the iso-C5/C5 ratio. The severity of dysbiosis increased with the severity of clinical forms of OLP. Changes in the qualitative and quantitative contents of SCFAs reflect the disturbances in gut microbiocenosis in LPO patients, which may be one cause for aggravation of the pathological process.
- Roopashree MR, Gondhalekar RV, Shashikanth MC, George J, Thippeswamy SH, Shukla A. Pathogenesis of oral lichen planus: a review. J Oral Pathol Med. 2010;39(10):729–34.
- O’Hara AM, Shanahan F. The gut flora as a forgotten organ. EMBO Rep. 2006;7(7):688–93
- Diaz Heijtz R, Wang S, Anuar F, Qian Y, Björkholm B, Samuelsson A, et al. Normal gut microbiota modulates brain development and behavior. Proc Natl Acad Sci USA. 2011;108(7):3047–52.
- Vijay-Kumar M, Aitken JD, Carvalho FA, Cullender TC, Mwangi S, Srinivasan S, et al. Metabolic syndrome and altered gut microbiota in mice lacking Toll-like receptor 5. Science 2010;328(5975):228–31.
- Maslowski KM, Vieira AT, Ng A, Kranich J, Sierro F, YuD, et al. Regulation of inflammatory responses by gut microbiota and chemoattractant receptor GPR43. Nature. 2009;461(7268):1282–6.
- Huda-Faujan N, Abdulamir AS, Fatimah AB, Anas OM, Shuhaimi M, Yazid AM, Loong YY. The impact of the level of the intestinal short chain Fatty acids in inflammatory bowel disease patients versus healthy subjects. Open Biochem J. 2010;4:53–58.
- Vernia P, Caprilli R, Latella G, Barbetti F, Magliocca FM, Cittadini M. Fecal lactate and ulcerative colitis. Gastroenterology. 1988;95(6):1564–8.
- Murphy EF, Cotter PD, Healy S, Marques TM, O’Sullivan O, Fouhy F, et al. Composition and energy harvesting capacity of the gut microbiota: relationship to diet, obesity and time in mouse models. Gut. 2010;59(12):1635–42.
- Schwiertz A, Taras D, Schafer K, Beijer S, Bos NA, Donus C, Hardt PD. Microbiota and SCFA in lean and overweight healthy subjects. Obesity. 2010;18(1):190–5.
- Simark-Mattsson C, Eklund S. Reduced immune responses to purified protein derivative and Candida albicans in oral lichen planus. J Oral Pathol Med. 2013;42(9):691-7.
- Petrova LV. Clinical features of lichen planus of the oral mucosa. Ross Zh Kozh Vener Bolezney. 2002;3:28-31.[Article in Russian].
- Cummings JH, Pomare EW, Branch WJ, Naylor CP, Macfarlane GT. Short chain fatty acids in human large intestine, portal, hepatic and venous blood. Gut. 1987;28(10):1221–7.
- McNeil NI. The contribution of the large intestine to energy supplies in man. Am J Clin Nutr.1984;39:338–42.
- Zaibi MS, Stocker CJ, O’Dowd J, Davies A, Bellahcene M, Cawthorne MA, et al. Roles of GPR41 and GPR43 in leptin secretory responses of murine adipocytes to short chain fatty acids. FEBS Lett. 2010; 584(11):2381–6.
- Plaisancie P, Dumoulin V, Chayvialle JA, Cuber JC. Luminal peptide YY-releasing factors in the isolated vascularly perfused rat colon. J Endocrinol. 1996;151(3):421–9.
- Vinolo MA, Rodrigues HG, Hatanaka E, Sato FT, Sampaio SC, Curi R. Suppressive effect of short-chain fatty acids on production of proinflammatory mediators by neutrophils. J Nutr Biochem. 2011; 22(9):849–55.
- Vinolo MA, Rodrigues HG, Hatanaka E, Hebeda CB, Farsky SH, Curi R. Short-chain fatty acids stimulate the migration of neutrophils to inflammatory sites. Clin Sci. 2009;117(9):331–8.
- Vinolo MA, Rodrigues HG, Nachbar RT, Curi R. Regulation of Inflammation by Short Chain Fatty Acids. Nutrients. 2011; 3(10): 858–76.
- Kraft R, Usatine RP. Lichen planus. In: The Color Atlas of Family Medicine. Usatine RP, Smith MA, Chumley H, Mayeaux EJ Jr., Tysinger J, eds. New York, NY: McGraw-Hill; 2009:634–639.
- Beleva NS, Libik TV. Factors systemic risk in patients with manifestations of lichen planus of the oral mucosa. Proceedings of the All-Russian scientific-practical conference of students, graduate students and young scientists. Perm, 2009:191-4. [Article in Russian].
- Surdina ED. Modern conceptions of the leading factors in development and treatment of lichen planus with manifestations in the oral mucosa. Vestnik St. Petersburg University. Ser.11. 2011;4:112-118. [Article in Russian].
- Oskolsky GI, Zagorodnaya EB. Role of dysbiotic shifts in patients with lichen planus of the oral mucosa. In: Actual problems and prospects of development of dentistry in the North. Ushnitskii ID, ed. Yakutsk, NEFU; 2011:170-174. [Article in Russian].
- Ron GI, Epishova AA. Modern ideas about the etiology and pathogenesis of lichen planus of the oral mucosa (review). Problemi Stomatol. 2011;4:15-17. [Article in Russian].
- Slujaev IF, Oskolsky GI, Zagorodnaya EB. Oral lichen planus of the oral mucosa: clinical features, treatment. Dal’ne-Vostochniy Med Zh. 2010;2:132-136. [Article in Russian].
- Pourshahidi S, Fakhri F, Ebrahimi H, Fakhraei B, Alipour A, Ghapanchi J, Farjadian S. Lack of association between Helicobacter pylori infection and oral lichen planus. Asian Pac J Cancer Prev.2012;13(5):1745-7.
- Taghavi Zenouz A, Mehdipour M, Jafari Heydarlou M, Gholizadeh N. Relationship between lichen planus and Helicobacter pylori infection. J Dent Res Dent Clin Dent Prospect. 2010;4(1):17-20.
- Ardatskaya MD, Dmitrieva LA, Khubutia BN, Georgieva OA, Osipov TL. The study of oral microflora metabolites (short chain fatty acids) in patients with lichen planus; diagnostic and tactical importance. Estestv Tekhnich Nauki. 2012;6(62):194-204. [Article in Russian].
- Niederman R, Buyle-Bodin Y, Lu BY, Robinson P, Naleway C. Short-chain carboxylic acid concentration in human gingival crevicular fluid. J Dent Res. 1997;76(1):575–9.
- Halili MA, Andrews MR, Labzin LI, Schroder K, Matthias G, Cao C, et al. Differential effects of selective HDAC inhibitors on macrophage inflammatory responses to the Toll-like receptor 4 agonist LPS. J Leukoc Biol. 2010;87(6):1103–14.
- Bailon E, Cueto-Sola M, Utrilla P, Rodriguez-Cabezas ME, Garrido-Mesa N, Zarzuelo A, et al. Butyrate in vitro immune-modulatory effects might be mediated through a proliferation-related induction of apoptosis. Immunobiology. 2010;215(11):863–73.
- Huuskonen J, Suuronen T, Nuutinen T, Kyrylenko S, Salminen A. Regulation of microglial inflammatory response by sodium butyrate and short-chain fatty acids. Br J Pharmacol. 2004;141:874–80.
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