Abstract OR-25: Fitting Structural Fragments into Electron Microscopy Density Maps Using Spectral Shape Descriptors

Amihai Holzer, Dina Schneidman-Duhovny

 
International Journal of Biomedicine.2019;9  Suppl_1:S16-S16.
http://dx.doi.org/10.21103/IJBM.9.Suppl_1.OR25
Originally published June 29, 2019  

Abstract: 

Background: Macromolecular assemblies play a major role in cellular processes. Atomic resolution structural characterization of these machineries is necessary for understanding and modulating their function. The resolution revolution in cryo-EM paved a way for solving structures that for years resisted X-ray crystallography. This progress introduced a need for a new generation of computational methods for automatic structure determination with high and medium resolution cryo-EM density maps.
Methods: We present a novel method for rapid fitting of structural fragments into high and medium resolution density maps. The fragments can range in size from peptides to large proteins. The method utilizes spectral Heat Kernel Signature (HKS) shape descriptors, that are used in 3D shape recognition in Computer Vision. The fragment descriptors are fitted to the descriptors of the density map using the Geometric Hashing approach.
Results: We benchmark the method on a set of maps with resolved atomistic structures in the 3.5-6Å resolution range (Fig.1).
Conclusion: The method is an important building block towards automated ab initio structure modeling.

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Keywords: 
atomic resolution structure modeling • EM density map fitting