Increase in the Synthesis of Polyfructan in the Cultures of Chicory “Hairy Roots” with Plant Natural Growth Regulators

BIOTECHNOLOGY
Victoria А. Tsygankova, PhD¹*, Alla I. Yemets, PhD, ScD², Sergey P. Ponomarenko, PhD, ScD³, Nadezhda A. Matvieieva, PhD", Sergey E. Chapkevich", Nikolay V. Kuchuk, PhD, ScD".

¹Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine,  Kyiv, Ukraine

²Institute of Food Biotechnology and Genomics, NAS of Ukraine, Kyiv, Ukraine

³National Enterprise Interdepartmental Science and Technology Center "Agrobiotech", NAS of Ukraine and MESS of Ukraine, Kyiv, Ukraine

"Institute of Cell Biology and Genetic Engineering, NAS of Ukraine, Kyiv, Ukraine

*Corresponding author: Victoria А. Tsygankova, PhD. Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine. Kyiv, Ukraine. E-mail: vtsygankova@ukr.net

Published: June 25, 2013

Abstract: 

Experiments were conducted to study the benefit of using the new plant growth regulators (PGRs) Ivin, Emistim, Biolan and Charkor in nutrient medium ½ MS for intensification of biomass growth and the increased synthesis of polyfructan (PF) in the cultures of chicory “hairy roots” (Cichorium intybus L.), obtained by Аgrobacterium rhizogenes-mediated transformation,. The best indexes of increased specific quantities of PF are observed after using Biolan at a concentration of 5.0 μL/L (up to 130 mg/g dry mass of roots) and Emistim at a concentration 2.5 µL/L (up to 220 mg/g dry mass of roots). The greatest stimulation of root growth activity was expressed on using the growth regulators Emistim, Ivin and Charkor, in concentrations between 2.5 and 10.0 µL/L, considerably raising the total quantity of PF: compared with the control the use of regulator Emistim showed a rise of up to 35 times, regulator Ivin showed an increase of up to 28 times and regulator Charkor showed an increase up to 7.0-7.5 times. The results thus obtained definitely prove the benefit of applying these regulators to increase the biomass growth and PF synthesis in the culture of chicory «hairy roots».

Keywords: 
plant growth regulators; chicory "hairy roots"; polyfructan.
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Int J Biomed. 2013; 3(2):139-144. © 2013 International Medical Research and Development Corporation. All rights reserved.