Association between ACE Gene I/D Polymorphism and Unstable Angina in Uzbek Patients with Family History of Coronary Heart Disease
Republican Specialized Center of Cardiology , Tashkent, Uzbekistan
*Corresponding author: Feruza M. Bekmetova, PhD, Department of Coronary Heart Disease, Republican Specialized Center of Cardiology, 4, Osiyo str., 100052, Tashkent, Uzbekistan. Tel: 998-90-3276814. E-mail: firstname.lastname@example.org
Objective: To study the distribution of I/D polymorphic marker of the ACE gene in Uzbek patients with unstable angina, depending on the presence of family history of CHD compared with healthy individuals.
Materials and methods: There were examined 125 Uzbek patients with unstable angina (class IIB by E. Braunwald et al., 1989). In patients with unstable angina 63 patients (the 1st group) had and 62 patients (the 2nd group) had no a family history of CHD. Control group included 45 healthy Uzbek subjects without CHD and family history of CHD. The genomic DNA was isolated from the peripheral blood lymphocytes following standard protocol using the DiatomTM DNA Prep 200 kit (produced by LLC "Laboratory IsoGene"). I/D polymorphism of the ACE gene was detected according to Cambian F. et al. (1992).
Results: In a comparative analysis of both groups, patients showed similarity in the baseline clinical, hemodynamic and biochemical parameters; however, the 1st group had significant higher IMC thickness of the carotid arteries and hsCRP levels. Simultaneously, patients with a family history of CHD, compared with the healthy group of individuals, were noted to have a significantly higher prevalence of the D/D genotype [OR: 3.46 (95% CI: 1.18-8.11; P=0.035)] and "damaging" D-allele [OR: 2.47 (95% CI: 1.40-4.34; P=0.002)].
Conclusion: The presence of a family history of the coronary heart disease among Uzbek patients with unstable angina was associated with the higher frequency of the "damaging” D-allele of the ACE I/D gene polymorphism, accompanied by an increase in the thickness of the intima-media complex of the carotid arteries and level of high-sensitivity C-reactive protein.
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