Significance of the TLR Expression in the Cells of the Female Reproductive Tract of Patients with Chronic Endometritis Treated with Sodium Nucleospermate

Sergey N. Proshin ¹*, L.A. Saikovskaya ², I.V. Semenova², N.I.Tapilskaya², I.N.Vorobtsova²

¹North West State Medical University named after I.I Mechnikov, St. Petersburg, Russian Federation,

²The State Pediatric Medical Academy, St. Petersburg, Russian Federation

*Corresponding author: Sergey N. Proshin, Department of pharmacology, North West State Medical University named after I.I Mechnikov. 45/5, Piskarevsky str., 195067, St. Petersburg, Russian Federation. Tel: 7- 911- 9594109. E-mail:


Immunocytochemistry was performed on the cells taken from the ecto/endocervix and endometrium. The monoclonal antibodies produced against CD20, CD56 and TLR have been used in the immunocytochemistry. The frequency of the immunocytochemically positive cells was estimated before and after a course of sodium nucleospermate treatment, in women suffering from infertility. The real–time PCR for HPV of high oncogenic risk was also studied. After the course of the treatment, the frequency of CD20, and especially of CD56 positive cells, was found to decrease. The concomitant lowering of the HPV of high oncogenic risk quantity was determined by real–time PCR. Otherwise, the frequency of TLR4 and TLR9 positive cells was found to be elevated. The data presented here proved the assumption that sodium nucleospermate actively induces an innate immune response and eliminated factors inciting endometrial inflammation.

TLR; CD20; CD56; endometrium; cervix of uteri; sodium nucleospermate.

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Int J Biomed. 2012; 2(4):261-266. © 2012 International Medical Research and Development Corporation. All rights reserved.