Gamaleya Institute of Epidemiology and Microbiology, Moscow, Russian Federation
*Corresponding author: Yulia F. Gorskaya, PhD, ScD, Gamaleya Institute of Epidemiology and Microbiology, 18, Gamaleya str., 123098, Moscow, Russian Federation. Tel: 7-499-1935541 E-mail: Uliya.Gorskaya@nearmedic.ru
The cloning efficiency (CFE-F) of stromal precursor cells (CFU-F) in cultures of heterotopic transplant cells and the quantity of CFU-F in bone marrow transplants from old donors to old recipients (O-O group) has decreased almost eight times, compared with transplants where the bone marrow from young donors has been transplanted to young recipients (Y-Y group). However, in the O-Y group, the CFE-F and the quantity of CFU-F in the transplants increased more than three times compared with the O-O group, however, staying 2.5 times lower than the Y-Y group level. Regarding transplanting bone marrow from young donors to old recipients (Y-O), the CFE-F of transplants decreased approximately two times lower compared with the CFE-F in the Y-Y group. The CFE-F in the cultures of the spleen transplant cells and the amount of CFUF in the transplants of the O-O group have decreased four and six times, respectively, compared with the Y-Y group. However, if the spleen from the old mice was transplanted to young recipients (O-Y), the CFE-F in transplants was noted to increase almost seven times compared with the CFE-F in spleen transplants from old donors to old recipients (O-O), and exceeded the level of the Y-Y group. These data suggest that the age-related decrease in the CFE-F and the quantity of CFUF in the bone marrow are the results of both changes in the stromal tissue (expressed in real reduction in number of CFU-F), and the regulating action of the organism on stromal tissue. Age-related decrease of CFE-F and the amount of CFU-F in spleen could occur, mainly due to the regulating action of the organism.
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