HLA Typing and Chromosomal Aberrations Caused by Chemical Agents

CLINICAL RESEARCH
Gulnara S. Agsamova, PhD

Tashkent Medical Academy; Tashkent, Uzbekistan

*Corresponding author: Gulnara S. Agzamova, PhD. Head of Department of Occupational Diseases of Tashkent Medical Academy; Tashkent, Uzbekistan. E-mail: agzamova_gulya@mail.ru

Abstract: 

The aim of this research was to perform HLA typing and determine the chromosomal aberrations in patients with chronic lead and pesticide (organophosphates and organochlorines) toxicity.

Materials and Methods: Cytogenetic studies were performed on 105 study participants. Eighty-five patients with chronic professional toxicity were divided into two groups. Group 1 consisted of 40 patients with chronic lead toxicity; Group 2 consisted of 65 patients with chronic pesticide toxicity. Cytogenetic effects were estimated using a metaphase plates technique. Cytostatic effect of studied chemicals was evaluated using PHA stimulation index.  Distribution of HLA- antigens was studded in 30 patients with chronic viral hepatitis and 60 patients with work-related chronic liver disease.

Results: The frequency of chromosomal aberrations in peripheral blood lymphocytes was increased in persons with occupational exposure to pesticides. In 66.7% of workers exposed to lead, the response to PHA stimulation was very low. Our results showed that HLA- A2, A25, B16, B8, B22 antigens were determined statistically more frequently in patients with chronic liver toxicity; HLA-A2/B8, A2/B15, A25/B22 haplotypes increased the risk of liver toxicity.

Keywords: 
chronic lead and pesticide toxicity; chromosomal aberrations; HLA antigens
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IJBM 2014; 4(4) Suppl 1:S20-S22. © 2014 International Medical Research and Development Corporation. All rights reserved.