Effectiveness of Photodynamic Therapy in the Healing of Corneal Alkali Burn in Rats

F.A. Bakhritdinova, PhD, ScD; K. I. Narzikulova, PhD; S.S. Mirrakhimova, PhD; Akshay Khera

Tashkent Medical Academy, Tashkent, Uzbekistan

Corresponding author: Professor Fazilat A. Bakhritdinova, PhD, ScD. Head of Department of Ophthalmology, Tashkent Medical Academy, Tashkent, Uzbekistan. E-mail: bakhritdinova@mail.ru

Published: June 20, 2016.  DOI: 10.21103/Article6(2)_OA6


In this study, we investigated the effect of photodynamic therapy (PDT) on the healing of corneal alkali burns in rats. The experiment was performed on 50 adult non-linear rats. Depending on the intervention, the animals were divided into 5 equal groups with 10 animals in each group: Group 1 included rats with intact eyes (the control group) and Groups 2 through 5 were experimental groups with EAB. Group 2 consisted of rats subjected to instillation of 0.25% chloramphenicol solution; Group 3 consisted of rats subjected to photodynamic irradiation according to our scheme: 300 mJ (630 nm) for 3 minutes; Group 4 consisted of rats subjected to instillation of methylene blue (MB); Group 5 consisted of rats subjected to instillation of MB with subsequent photodynamic irradiation according to the described  scheme.

During all periods of observation, the infiltration of the subcorneal zone was less pronounced in Group 5 than in the other groups and was represented mainly by round cells in the anterior chamber, iris, retina, and ciliary zone. The instillation of MB with subsequent photodynamic irradiation was the most effective in reducing the bacterial contamination

 Thus, PDT with the photosensitizer methylene blue, in accordance with the designed exposure mode, provided the epithelialization and bacteriostatic effect during corneal repair after EAB. In conclusion, PDT improves a wound’s healing process, which is expressed in the reduction of inflammatory infiltration and the promotion of corneal epithelialization.

ocular surface; experimental alkali burn; photodynamic therapy; methylene blue.
  1. Ochirova EK, AN Plekhanov AN. Drug treatment of eye burns: review. Bulletin of ESSC SB RAMS. 2010; 3:366-69.
  2. Maychuk YF Optimization of pharmacotherapy for inflammatory diseases of eye surface. ROJ. 2008; 3:18-25
  3. Zolotarev AV, Malyshev AC, Fadeeva AV, Morozova IuV, Podsevakina TA.  Photodynamic visudyne therapy for subretinal neovascularization. Vestn Oftalmol. 2007; 123(6): 21-3.
  4. Bakhritdinova FA, Narzikulova KI, Mirrakhimova SSh. Effect of photodynamic therapy on the rate of endogenous intoxication. J Ophthalmol (Ukraine) 2015; 4(465):35-7.
  5. Bakhritdinova FA, Narzikulova KI,  Mirrakhimova SSh.  Study of the Efficacy of Photodynamic Therapy in the Experiment The 7th International Conference on Ocular Infections. Barselona. 2015; p.52.
  6. Yoon KC, You IC, Kang IS, Im SK, Ahn JK, Park YG, Ahn KY. Photodynamic therapy with verteporfin for corneal neovascularization. Am J Ophthalmol. 2007; 144(3):390-5.
  7. Sadykov RA, Kasimova KR, Sadykov RR. Technical and scientific aspects of the photodynamic therapy. Tashkent; 2012 . [Manual in Russian].
  8. Eter N, Spaide RF. Comparison of fluorescein angiography and optical coherence tomography for patients with choroidal neovascularization after photodynamic therapy. Retina. 2005; 25(6):691-6.
  9. Donaldson MJ, Lim L, Harper CA, Mackenzie J, Campbell WG. Primary treatment of choroidal amelanotic melanoma with photodynamic therapy. Clin Experiment Ophthalmol. 2005;33(5):548-9.
  10. Tatar O, Adam A, Shinoda K, Yoeruek E, Szurman P, Bopp S, et al. Influence of verteporfin photodynamic therapy on inflammation in human choroidal neovascular membranes secondary to age-related macular degeneration.  Retina. 2007; 27(6):713-23.
  11. Avetisov SE, Budzinskaia MV, Kiseleva TN, Kazarian EE, Gurova IV, Loshchenov VB, et al. Photodynamic therapy in treatment of chorioid neovascularization.  Vestn Ross Akad Med Nauk. 2007;(8):45-8.
  12. Izmailov AS, Balashevich LI.  Photodynamic therapy with visudyne: criteria for treatment success. Vestn Oftalmol. 2007; 123(6):17-21.
  13. Belyi YA, Tereshchenko AV, Volodin PL, Ivanov AM, Shatskikh AV. Experimental studies of photodynamic effects in the prevention of complications after removing intraocular foreign bodis impacted into the posterior pole of the eye. Vest OSU. 2007; S12: 15-18.
  14. Hamblin MR, Hasan T. Photodynamic therapy: a new antimicrobial approach to infectious disease? Photochem Photobiol Sci. 2004 May;3(5):436-50.
  15. Kanyukov VN, Stadnikov AA Trubina, OM, Yakhina OM. Experimental modeling of traumatic injuries of the cornea. Vest OSU. 2014; 12 (173):156-9. [Article in Russian].
  16. Obenberger J. New methods for experimental studies of the anterior eye segment using strips and rings prepared from chromatographic paper. Cesk Oftalmol. 1975; 31(3):201-8. [Article in Czech]
  17. Kassens M, Wegerhoff R, Wheidlich O. Basics of Light Microscopy and Imaging. GIT Verlag GmbH and Co.KG; 2006.
  18. Garib FY, Norbaeva IE, Bektimirov AM. Microbiological methods. Tashkent; 1994. [Guidelines in Russian].
  19. Moan J, Peng Q. An outline of the hundred-year history of PDT. Anticancer Res. 2003;23:3591–3600
  20. Malik Z, Ladan H, Nitzan Y. Photodynamic inactivation of Gram-negative bacteria: problems and possible solutions. J. Photochem. Photobiol., B. 1992;14:262–266. [PubMed]
  21. Dai T1, Huang YY, Hamblin MR. Photodynamic therapy for localized infections--state of the art. Photodiagnosis Photodyn Ther. 2009 Sep-Dec;6(3-4):170-88.
  22. Lambrechts SA, Demidova TN, Aalders MC, Hasan T, Hamblin MR. Photodynamic therapy for Staphylococcus aureus infected burn wounds in mice.Photochem Photobiol Sci. 2005; 4(7):503-9.
  23. Cook N. Methicillin-resistant Staphylococcus aureus versus the burn patient. Burns.1998;24(2):91-8.

The fully formatted PDF version is available.

Download Article

Int J Biomed. 2016;6(2):124-127. © 2016 International Medical Research and Development Corporation. All rights reserved.