Antidiarrheal Activity of Three Medicinal Plants in Swiss Albino Mice

MD. Ashrafuzzaman, PhD¹; Golam Sarwar Raju²; Muhammad Imam Uddin Abeer²; Saifuddin Mohammad Seeam²; Abu Zafor Mohammad Salahuddin¹; Dipti Debnath³; H.M. Shadid Hossain Snigdha^; Bhorhan Uddin Sawan²; S. M. Masud Rana¹*

¹Department of Biomedical Engineering, Military Institute of Science and Technology (MIST), Dhaka, Bangladesh; ²Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh; ³Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka, Bangladesh; ^ Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh

*Corresponding Author: S. M. Masud Rana. Department of Biomedical Engineering, MIST, Dhaka, Bangladesh; E- mail:

Published: September 12, 2016.  DOI: 10.21103/Article6(3)_OA16


Background: Different parts of Allamanda neriifolia (AN), Crinum latifolium (CL), and Bruguiera cylindrical (BC) are used in folk medicine to treat diarrhea. Therefore, the aim of this study was to investigate and compare possible antidiarrheal activity of methanol extracts from barks, stems, and roots of AL, CL, and BC in Swiss albino mice.
Methods: Antidiarrheal activities of extracts were evaluated at three doses (100mg/kg, 200 mg/kg and 400mg/kg) and compared with Loperamide in a castor oil-induced diarrhea and charcoal meal test model in the Swiss albino mice.
Results: The aqueous extract of CL and BC administered at doses of 100, 200 and 400mg/kg showed 0%, 24.5%, 62.26% and 5.66%, 37.11%, and 62.26% diarrhea inhibition, respectively (Table 2). This reduction in diarrheal episodes is significant, and maximum effect was observed at the dose of 400mg/kg similarly in the alcohol extracts of both CL and BC. AN administered at the dose of 100, 200 and 400mg/kg showed 55.97%, 74.84% and 74.84% diarrhea inhibition, respectively.
Conclusion: The antidiarrheal effect of the AN extract, in contrast to CL and BC, against the castor oil-induced diarrhea model prove its efficacy in an extensive range of diarrheal conditions.

Allamanda neriifolia; Crinum latifolium; Bruguiera cylindrical; antidiarrheal activity.
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Int J Biomed. 2016;6(3):233-236. © 2016 International Medical Research and Development Corporation. All rights reserved.