¹Department of Biomedical Engineering, Military Institute of Science and Technology (MIST), Dhaka, Bangladesh; ²Department of Pharmacy, Noakhali Science and Technology University, Noakhali, Bangladesh; ³Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka, Bangladesh; ^ Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh
*Corresponding Author: S. M. Masud Rana. Department of Biomedical Engineering, MIST, Dhaka, Bangladesh; E- mail: firstname.lastname@example.org
Published: September 12, 2016. DOI: 10.21103/Article6(3)_OA16
Background: Different parts of Allamanda neriifolia (AN), Crinum latifolium (CL), and Bruguiera cylindrical (BC) are used in folk medicine to treat diarrhea. Therefore, the aim of this study was to investigate and compare possible antidiarrheal activity of methanol extracts from barks, stems, and roots of AL, CL, and BC in Swiss albino mice.
Methods: Antidiarrheal activities of extracts were evaluated at three doses (100mg/kg, 200 mg/kg and 400mg/kg) and compared with Loperamide in a castor oil-induced diarrhea and charcoal meal test model in the Swiss albino mice.
Results: The aqueous extract of CL and BC administered at doses of 100, 200 and 400mg/kg showed 0%, 24.5%, 62.26% and 5.66%, 37.11%, and 62.26% diarrhea inhibition, respectively (Table 2). This reduction in diarrheal episodes is significant, and maximum effect was observed at the dose of 400mg/kg similarly in the alcohol extracts of both CL and BC. AN administered at the dose of 100, 200 and 400mg/kg showed 55.97%, 74.84% and 74.84% diarrhea inhibition, respectively.
Conclusion: The antidiarrheal effect of the AN extract, in contrast to CL and BC, against the castor oil-induced diarrhea model prove its efficacy in an extensive range of diarrheal conditions.
- World Health Organization. Diarrhoeal Diseases Control Programme: interim programme report 1988 (WHO/CDD/89.31). http://apps.who.int/iris/bitstream/10665/126857/1/sea_achr_15_inf.9.Pdf
- Saralaya MG, Patel P, Patel M, Roy SP, Patel AN. Anti-diarrhea activity of methanolic extract of Moringa oleifera Lam roots I experimental animal models. Int J Pharm Res. 2010; 2(2): 35-39.
- Toyin YM, Khadijat OF, Saoban SS, Olakunle AT, Abraham BF, LuqmanQA. Antidiarrheal activity of aqueous leaf extract of Ceratotheca sesamoides in rats. Bangladesh J Pharmacol 2012; 7: 14– 20.
- Krause R, Schwab E, Bachhiesl D, Daxböck F, Wenisch C, Krejs GJ, Reisinger EC. Role of Candida in antibiotic-associated diarrhea.J Infect Dis. 2001;184(8):1065-9.
- Maresca M, Mahfoud R, Garmy N, Kotler DP, Fantini J, Clayton F. The virotoxin model of HIV-1 enteropathy: involvement of GPR15/Bob and galactosylceramide in the cytopathic effects induced by HIV-1 gp120 in the HT-29-D4 intestinal cell line. J Biomed Sci. 2003 Jan-Feb;10(1):156-66.
- Jimba Y, Nagao J, Sumiyama Y. Changes in gastrointestinal motility after subtotal colectomy in dogs. Surg Today. 2002;32(12):1048-57.
- Qnais EY, Abdulla FA, Abu Ghalyn YY. Antidiarrheal effects of Juniperus phoenicia L. leaves extracts in rats. Pakistan Journal of Biological Sciences 2005; 8(6): 867-71
- Hejazian SH. Morowatisharifabad M, Mahdavi SM. Relaxant effect of Carum copticum on intestinal motility in ileum of rat. World Journal of Zoology. 2007; 2(2): 15-18
- Galvez J, Zavezuelo A, Crespo ME, Lorente MD, Ocete MA, Jimenez J. Antidiarrhoeic activity of Euphorbia hirta extract and isolation of an active flavonoid constituent. Planta Med. 1993; 59(4):333-6.
- Gorard DA, Libby GW, Farthing, MJ. Ambulatory small intestinal motility in 'diarrhoea' predominant irritable bowel syndrome. Gut 1994; 35(2): 203-10.
- Akomolafe RO, Adeoshun IO, Elujoba AA, Iwalewa EO, Ayoka AO. In vitro effects of Cassia sieberiena leave extracts on the intestinal motility of rats. African Journal of Biomedical Research. 2003; 6(1):141-5.
- Capasso F, Mascolo N, Izzo AA, Gaginella TS. Dissociation of castor oil-induced diarrhoea and intestinal mucosal injury in rat: effect of NG-nitro-L-arginine methyl ester. Br J Pharmacol. 1994; 113(4):1127-30.
- Chitme HR, Chandra M, Kaushik S. Studies on anti-diarrhoeal activity of Calotropis gigantea R.Br. in experimental animals. J Pharm Pharm Sci. 2004 Feb 25;7(1):70-5.
- Das AK, Rohini R, Hema A. Evaluation of anti-diarrhoeal activity of Rhizophora mucronata bark extracts. The Internet Journal of Alternative Medicine 2008; 7(1). http://print.ispub.com/api/0/ispub-article/3532.
- Uddin SJ, Shilpi JA, Alam SM, Alamgir M, Rahman MT, Sarker SD. Antidiarrhoeal activity of the methanol extract of the barks of Xylocarpus moluccensis in castor oil- and magnesium sulphate-induced diarrhoea models in mice. J Ethnopharmacol. 2005 Oct 3;101(1-3):139-43
- Awouters F, Niemegeers CJ, Lenaerts FM, Janssen PA. Delay of castor oil diarrhoea in rats: a new way to evaluate inhibitors of prostaglandin biosynthesis. J Pharm Pharmacol. 1978; 30(1):41-5
- Meite S, N’guessan JD, Bahi C, Yapi HF, Djaman AJ, Guede-Giuna F. Antidiarrhoeal activity of the ethyl acetate extract of Morinda morindoides in rats. Tropical Journal of Pharmaceutical Research. 2009; 8(3): 201-207
- Oben JE, Assi SE, Agbor GA, Musoro DF. Effect of Eremomastax speciosa on experimental diarrhea. African Journal of Traditional, Complementary and Alternative Medicine 2006; 3(1): 95-100
- Roe DA. Diet and Drug Interactions. Chapter 6: Drug-induced nutritional deficiencies. Springer; 1989, pp. 83-103
- Pierce NF, Hirschhorn N. Oral fluid: a simple weapon against dehydration in diarrhoea: how it works and how to use it. WHO chronicle 1977; 31(3):87-93
- Zia-Ul-Haq M, Shahid SA, Muhammed S, Qayum M, Khan I, Ahmad S. Antimalarial, antiemetic and antidiabetic potential of Grewia aslatica L. leaves. Journal of Medicinal Plants Research 2012; 6(16):3087-92.
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