Swelling and Degradation of Calcium-Pectic Gel Particles Made of Pectins of Silene vulgaris and Lemna minor Сallus Cultures at Different Concentrations of Pectinase in an Artificial Colon Environment

Anatoly A. Shubakov, PhD¹; Elena A. Mikhailova¹,²*; Valentina I. Prosheva, PhD¹; Vladimir A. Belyy, PhD³

¹Institute of Physiology, Komi Science Centre, Ural Branch of the RAS; ²Komi Republic Research Institute of Agriculture, Komi Science Centre, Ural Branch of the RAS; ³Institute of Chemistry, Komi Science Centre, Ural Branch of the RASSyktyvkar, Komi Republic, the Russian Federation

*Corresponding author: Elena A. Mikhailova. Department of Molecular Immunology and Biotechnology, Institute of Physiology, Komi Science Centre, Ural Branch of the RAS. Syktyvkar, the Russian Federation. E-mail: elena_elkina@mail.ru

Published: March 15, 2018.  doi: 10.21103/Article8(1)_OA10

Abstract: 

Spherical calcium-pectic gel particles (CaPGPs) were obtained from pectins of callus cultures (CC) of campion Silene vulgaris (silenan) and duckweed Lemna minor (lemnan), as well as from commercial apple and citrus pectins by the method of ionotropic gelation. We studied the morphological characteristics of the obtained gel particles and found that the largest gel particles were formed from citrus pectin (CP), and the densest gel particles were formed from lemnan pectin (LP). The swelling and degradation of CaPGPs were comparatively evaluated by incubation in a simulated gastrointestinal environment. The swelling and degradation of CaPGPs formed from pectins of the silenan and lemnan callus cultures were studied at different concentrations of pectinase (0.4, 0.8 and 1.7 mg/ml) in the simulated fluid of the colon. It has been established that the CaPGPs obtained from lemnan are more resistant to degradation in the simulated colon fluid than CaPGP obtained from silenan. It was shown that the concentration of pectinase in the simulated fluid of the colon and the type of pectin affect the degradability of CaPGPs formed on the basis of pectins of callus cultures.

Keywords: 
pectins ● silenan ● lemnan ● callus culture ● gel particles ● gastrointestinal tract ● colon ● concentration of pectinase
References: 

1. Kushak RI, Drapeau C, Winter HS. Pancreatic and intestinal enzyme activities in rats in response to balanced and unbalanced plant diets. Plant Foods Hum Nutr. 2002;57(3-4):245-55.
2. Macfarlane S, McBain AJ, Macfarlane GT. Consequences of biofilm and sessile growth in the large intestine. Adv Dent Res. 1997;11(1):59-68.
3. Ovodov IuS. [Current information about pectin substances]. Bioorg Khim. 2009;35(3):293-310. [Article in Russian].
4. Patova OA, Golovchenko VV, Ovodov YuS. Pectic polysaccharides: structure and properties. Russ Chem Bull. 2014;63(9):1901-24.
5. Morris G, Kők S, Harding S, Adams G. Polysaccharide drug delivery systems based on pectin and chitosan. Biotechnol Genet Eng Rev. 2010;27:257-84.
6. Günter EA, Popeyko OV, Markov PA, Martinson EA, Litvinets SG, Durnev EA, et al. Swelling and morphology of calcium pectinate gel beads obtained from Silene vulgaris callus modified pectins. Carbohydr Polym. 2014;103:550-7. doi: 10.1016.j.carbpol.2013.12.071
7. Gűnter EA, Popeyko OV. Calcium pectinate gel beads obtained from callus cultures pectins as promising systems for colon-targeted drug delivery. Carbohydr Polym. 2016;147:490-9. doi: 10.1016/j.carbpol.2016.04.026
8. Dongowski G, Lorenz A, Proll J. The degree of methylation influences the degradation of pectin in the intestinal tract of rats and in vitro. J Nutr. 2002;132(7):1935-44.
9. Dongowski G, Lorenz A, Anger H. Degradation of pectins with different degrees of esterification by Bacteroides thetaiotaomicron isolated from human gut flora. Appl Environ Microbiol. 2000;66(4):1321-7. 
10. Sriamornsak P, Nunthanid J. Calcium pectinate gel beads for controlled release drug delivery: I. Preparation and in vitro release studies. Int J Pharm. 1998;160:207-12.
11. Sriamornsak P. Effect of calcium concentration, hardening agent and drying condition on release characteristics of oral proteins from calcium pectinate gel beads. Eur J Pharm Sci. 1999;  8(3):221-7.
12. Gebara C, Chaves KS, Ribeiro MCE, Souza FN, Grosso CRF, Gigante ML. Viability of Lactobacillus acidophilus La5 in pectin-whey protein microparticles during exposure to simulated gastrointestinal conditions. Food Res Int. 2013; 51(2):872-8. doi: 10.1016/j.foodres.2013.02.008
13. Oliveira GF, Ferrari PC, Carvalho LQ, Evangelista RC. Chitosan-pectin multiparticulate systems associated with enteric polymers for colonic drug delivery. Carbohydr Polym. 2010;82(3):1004-09. doi: 10.1016/j.carbpol.2010.06.041
14. Günter EA, Popeyko OV, Istomina EI. Effect of carbohydrates on the composition and gel-forming properties of callus pectins Silene vulgaris. Chemistry of Natural Compounds. 2015;51:409-414.
15. Matveyev YuI. A method of calculating gelation parameters in aqueous solutions of biopolymers. Vysokomolekulyarnye Soedinenija.  Seriya B. 2003;45(5):841-850.
16. Mikhailova EA, Melekhin AK, Belyy VA, Shubakov AA. Stability of hyaluronan-pectic gel particles in the conditions of the artificial gastrointestinal environment. International Journal of Biomedicine. 2017;7(4):310-314. doi: 10.21103/Article7(4)_OA8
17. Sriamornsak P., Kennedy R.A. Swelling and diffusion studies of calcium polysaccharide gels intended for film coating. Int J Pharm. 2008; 358(1-2):205-13. doi: 10.1016/j.ijpharm.2008.03.009.
18. Popov SV, Markov PA, Patova OA, Vityazev FV, Bakutova LA, Borisenkov MF, et al. In vitro gastrointestinal-resistant pectin hydrogel particles for β-glucuronidase adsorption. J Biomater Sci Polym Ed. 2017;28(3):293-311. doi: 10.1080/09205063.2016.1268461
19. Englyst HN, Hay S, Macfarlane GT. Polysaccharide breakdown by mixed populations of human faecal bacteria. FEMS Microbiol Ecol.  1987;95:163-171.
20. Salyers AA. Energy sources of major intestinal fermentative anaerobes. Am J Clin Nutr. 1979;32(1):158-63.
 

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International Journal of Biomedicine. 2018;8(1):60-64. ©2018 International Medical Research and Development Corporation. All rights reserved.