Cytokine Gene Polymorphisms in Chronic Adenoiditis

Natalia V. Terskova, Natalia A. Shnayder, Andrey S. Simbirtsev, Sergey G. Vakhrushev, Dinara R. Sidorenko, Natalia V. Platonova

International Journal of Biomedicine. 2018;8(3):213-216.   
DOI: 10.21103/Article8(3)_OA8             
Originally published September 15, 2018  


The aim of our research was to study the multiphase response in a system of pro-inflammatory and anti-inflammatory cytokines due to the additive contribution of homozygous and heterozygous genotypes for the polymorphic allelic variants of the interleukin-1β (IL-1β) and interleukin-4 (IL-4) genes in patients with chronic adenoiditis (CA).
Materials and Methods: The study included 388 children with CA. Associations between the IL1B gene (rs1143634) (C+3954T) SNP and the IL-4 gene (rs2243250) (C-589T) SNP and the clinical manifestations and clinical outcome of CA were investigated. Genotyping for the studied SNPs was performed using real-time PCR. The study of genotype-associated cytokine production in accordance with the level of concentration of IL-1β, IL-4 in blood serum with the method of solidphase EIA using horseradish peroxidase as an indicating enzyme was carried out.
Results: The presence of homozygous or heterozygous genotypes of the studied SNPs of the IL-1β and IL-4 genes was characterized with genetically determined cytokine-production forming the phenotypical polymorphism. The conducted research into congenital immunity factors with an assessment of genetically determined cytokine production has revealed 5 options of the cytokine response and their corresponding frequencies. We extrapolated the results on clinical and functional outcomes of chronic adenoiditis, which allowed us to identify non-randomness in the nature of chronic adenoiditis as a multifactorial disease.
Conclusion: The obtained data are evidence of the phenotypic-genetic heterogeneity of CA.

chronic adenoiditis • interleukin-1β • interleukin-4 single nucleotide polymorphism
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Received April 10, 2018.
Accepted July 16, 2018.
©2018 International Medical Research and Development Corporation.