Pharmacologically Active Tripeptide Leu-Ile-Lys in Indomethacin-Induced Gastric Ulcer

Aleksandr Yu. Zharikov, Samira E. Lorents, Igor P. Bobrov, Olesya N. Mazko, Olesya G. Makarova

International Journal of Biomedicine. 2018;8(4):351-354.   
DOI: 10.21103/Article8(4)_OA17
Originally published December 15, 2018  


The aim of the study was to examine the effect of a new tripeptide, Leu-Ile-Lys, on an experimental indometacin-induced gastric ulcer in rats.
Materials and Methods: The experiment was performed with 24 male Wistar rats (average weight of 150 g). Rats were randomly divided into 3 groups: Group 1(n=8) – the ulcer control group (IIGU), Group 2 (n=8) – the experimental group (IIGU+pre-treating with the tripeptide Leu-Ile-Lys), and Group 3 (n=8) – the comparison group (IIGU+pre-treating with omeprazole). The model of IIGU in rats was performed by a single intragastric administration of indomethacin (60mg/kg in 1ml of physiological saline). In Group 1, indomethacin caused the appearance of severe injuries of the mucosa with the presence of extensive edema and leukocyte infiltration in the submucosal layer. In animals of Group 2, which were pre-treated with the tripeptide Leu-Ile-Lys, macroscopically gastric mucosa also looked smooth and atrophic changes were not found. Destructive changes were not severe; they appeared only in the form of small spot erosions. The number of spot erosions was 2.6 times less than in Group 1. The average erosion depth was 6.8 times less than in Group 1, and 2.0 times less than in Group 3.
Conclusion: Results of this study demonstrated the high, comparable to the action of omeprazole, gastroprotective activity of the new tripeptide Leu-Ile-Lys.

peptic ulcer • Indometacine • oligopeptides • gastroprotective activity
  1. GBD 2013 Mortality and Causes of Death Collaborators. Global, regional, and national age-sex specific all-cause and cause-specific mortality for 240 causes of death, 1990-2013: a systematic analysis for the Global Burden of Disease Study 2013. Lancet. 2015;385(9963):117-71. doi: 10.1016/S0140-6736(14)61682-2. PubMed
  2. Martins JL, Rodrigues OR, da Silva DM, Galdino PM, de Paula JR, Romão W, et al. Mechanisms involved in the gastroprotective activity of Celtis iguanaea (Jacq.) Sargent on gastric lesions in mice. J Ethnopharmacol. 2014;155(3):1616-24. doi: 10.1016/j.jep.2014.08.006. PubMed
  3. Lanas A, Chan FKL. Peptic ulcer disease. Lancet. 2017;390(10094):613-624. doi: 10.1016/S0140-6736(16)32404-7. PubMed
  4. Lorents SE, Zharikov AY, Bobrov IP, Mazko ON, Makarova OG, Kiselyov VI. [Gastroprotective action of the peptide complex from pig kidneys at experimental «indomethacine» ulcer in rats]. Siberian Scientific Medical J. 2017;6(37):5-9. [Article in Russian].
  5. Zharikov AY, Zharikova GV, Mazko ON, Makarova OG, Kirjakova VO. [Identification of the issue of the new biologically active substances for the treatment of urolithiasis]. Bulletin of Medical Science 2017;1(5):28-31. [Article in Russian].
  6. Suzuki H, Hibi T. Oxidative stress in Helicobacter pylori-assocaited gastroduodenal disease. J Clin Biochem Nutr. 2006;39:56-63.
  7. Becker JC, Domschke W, Pohle T. Current approaches to prevent NSAID-induced gastropathy - COX selectivity and beyond. Br J Clin Pharmacol. 2004;58(6):587-600. PubMed
  8. Zharikova GV, Zharikov AYu, Kiselev VI Mazko ON, Makarova OG, Kirjakova VO. [The influence of the peptide complex of pig kidneys on the free radical oxidation by experimental urolithiasis]. Siberian Scientific Medical J 2017;2(37):17-21. [Article in Russian].
  9. Lorents SE, Zharikov AYu, Mazko ON, Makarova OG, Korenovsky YuV, Popovtseva AV, et al. [Influence of the peptide complex from pork kidneys tissues on indicators of free-radical oxidation and the expression of COX-1,2 in experimental gastropathy. Experimental gastroenterology]. 2018; 154(6):81-85. [Article in Russian].

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Received October 9, 2018.
Accepted November 11, 2018.
©2018 International Medical Research and Development Corporation.