Features of the Processes of Lipoperoxidation and Antioxidant Protection in the Pathogenesis of Infertility in Men of Different Ethnic Groups, Carriers of Nonfunctional Polymorphisms of the GSTT1 and GSTM1 Genes

N. A. Kurashova, T. A. Bairova, M. I. Dolgikh, B. G. Dashiev, L. I. Kolesnikovа

International Journal of Biomedicine. 2019;9(1):31-34.
DOI: 10.21103/Article9(1)_OA5
Originally published March 15, 2019


A comparative analysis was carried out of the parameters of lipoperoxidation and antioxidant protection in fertile and infertile men of two ethnic groups (Caucasians and Mongoloids), who were carriers of different genotypes of xenobiotic biotransformation genes (GSTT1 and GSTM1). In serum and ejaculate, we determined the content of thiobarbituric acid (TBA-AP), α-tocopherol, reduced glutathione (GSH) and oxidized glutathione (GSSG); the activity of glutathione-S-transferase (GST), glutathione peroxidase (GPO) and glutathione reductase (GR); and total antioxidant activity (AOA). It was found that in Caucasian fertile men, carriers of genotypes GSTT1(*0/*0)/GSTM1(*0/*0), there was a decrease in GST activity in serum and ejaculate with an increase in AOA in these substrates in comparison with similar indicators in carriers of genotypes GSTT1(1+/1+)/GSTM1(1+/1+). In Mongoloid fertile men, carriers of genotypes GSTT1(*0/*0)/GSTM1(*0/*0), in comparison with similar indices in carriers of genotypes GSTT1(1+/1+)/GSTM1(1+/1+), we found an increase in serum content of α-tocopherol and activity of GPO, a decrease in GST activity, and a decrease in the level of α-tocopherol in the ejaculate. In Caucasians with infertility, carriers of genotypes GSTT1(*0/*0)/GSTM1(0*/*0), we found decreased activity of GST in the blood and ejaculate. In this group, we also registered a decrease in the GSH level, with an increase in the level TBA-AP and GPO activity. In infertile Mongoloids, carriers of genotypes GSTT1(*0/*0)/GSTM1(*0/*0), we found an increase in the level TBA-AP and GPO activity and a decrease in serum α-tocopherol level.

oxidative stress • male infertility • GSTT1 • GSTM1

1. Bisht S, Faiq M, Tolahunase M, Dada R. Oxidative stress and male infertility. Nat Rev Urol. 2017;14(8):470-485. doi: 10.1038/nrurol.2017.69.
2. Kurashova NA, Dolgikh MI, Ershova OA, Gavrilova OA, Osipova EV, Dashiev BG, et al. Associations of polymorphic variants of the biotransformation genes with the components of the glutathione system in men with infertility. International Journal of Biomedicine 2017;3(7):226-230. doi: 10.21103/Article7(3)_OA13.
3. O’Flynn O’Brien KL, Varghese AC, Agarwal A. The genetic causes of male factor infertility: a review. Fertil Steril 2010;93(1):1–12. doi:10.1016/j.fertnstert.2009.10.045
4. Kolesnikova LI, Kolesnikov SI, Kurashova NA, Osadchuk LV, Osadchuk AV, Dolgikh MI, et al. Reproductive health and peculiarities of lipid peroxidation–antioxidant defense system in men of the main ethnic groups of the Baikal region. Bull Exp Biol Med. 2015;160(1):32-4. doi: 10.1007/s10517-015-3091-6
5. Kolesnikova LI, Kurashova NA, Bairova TA, Dolgikh MI, Ershova OA, Natyaganova LV, et sl. Features of lipoperoxidation, antioxidant defense, and thiol/disulfide system in the pathogenesis of infertility in males, carriers of nonfunctional variants of GSTT1 and GSTM1 gene polymorphisms. Bull Exp Biol Med. 2017;163(3): 378-380. doi: 10.1007/s10517-017-3808-9..
6. Agarwal A, Mulgund A, Sharma R, Sabanegh E. Mechanisms of oligozoospermia: an oxidative stress perspective. Syst Biol Reprod Med. 2014;60(4):206-16. doi: 10.3109/19396368.2014.918675.
7. Agarwal A, Cho CL, Esteves SC, Majzoub A. Development of treatment strategies in men with vulnerable sperm. Transl Androl Urol. 2017;6(Suppl 4):S476-S478. doi: 10.21037/tau.2017.04.28.
8. Discussion satellite symposium. Oxidative stress as a cause of male infertility. The management of the infertile couple. Effektivnaja farmakoterapija. 2014;(32):58-62. [In Russian].
9. Aitken RJ, Smith TB, Jobling MS, Baker MA & De Iuliis GN. Oxidative stress and male reproductive health. Asian J Androl. 2014;16(1):31-8. doi:10.4103/1008-682X.122203.
10. Darenskaya MA, Kolesnikov SI, Rychkova LV, Grebenkina LA, Kolesnikova LI. Oxidative stress and antioxidant defense parameters in different diseases: ethnic aspects. Free Radical Biology & Medicine. 2018;1(120):60. doi: 10.1016/j.freeradbiomed.2018.04.199
11. Safarinejad MR, Dadkhah F, Ali Asgari M, Hosseini SY, Kolahi AA, Iran-Pour E. Glutathione S-transferase polymorphisms (GSTM1, GSTT1, GSTP1) and male factor infertility risk: a pooled analysis of studies. Urol J. 2012;9(3):541-8.
12. Belyaeva EV, Yershova OA, Astahova TA, Bugun OV. [Glutathione S-transferase polymorphism in ethnic groups living in Eastern Siberia]. Vavilov Journal of Genetics and Breeding. 2017; 21(5):576-580. doi: 10.18699/VJ17.274. [Article in Russian].
13. Agarwal A, Virk G, Ong C, du Plessis SS. Effect of oxidative stress on male reproduction. World J Mens Health. 2014;32:1–17. doi: 10.5534/wjmh.2014.32.1.1.
14. Kolesnikova LI, Kolesnikov SI, Darenskaya MA, Grebenkina LA, Kolesnikova LR., Korytov LI, Suslikova MI. Oxidative stress as a mechanism of reduced glucose absorption under conditions of immobilization stress. Bull Exp Biol Med. 2017;164(2):132-135. doi: 10.1007/s10517-017-3941-5.
15. Trofimova T, Lizneva D, Suturina L, Walker W, Chen Yh, Azziz R, Layman LC. Genetic basis of eugonadal and hypogonadal female reproductive disorders. Best Pract Res Clin Obstet Gynaecol. 2017;44:3-14. doi: 10.1016/j.bpobgyn.2017.05.003.

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Received January 28, 2019.
Accepted February 20, 2019.
©2019 International Medical Research and Development Corporation.