Anti-Lithogenic Activity of Tetrapeptide Leu-Ile-Lys-His in Experimental Urolithiasis

N. N. Yakushev, A. Yu. Zharikov, O. N. Mazko, O. G. Makarova, G. V. Zharikova, V. M. Brukhanov, Yu. V. Korenovsky, A. V. Lepilov, I. P. Bobrov, O. V. Azarova

International Journal of Biomedicine. 2019;9(2):155-158.
DOI: 10.21103/Article9(2)_OA15
Originally published June 15, 2019  


Background: The research objective was to study the effect of the tetrapeptide Leu-Ile-Lys-His on the course of experimental oxalate nephrolithiasis.
Methods and Results: Experiments were conducted on 30 male Wistar rats, which were divided equally into 3 groups: the control group (6 weeks of ON modeling), the test group (6 weeks of ON modeling+the tetrapeptide Leu-Ile-Lys-His introduction at a dose of 12 mg/kg), and the experimental group (determination of indicators of free radical oxidation process activity in intact rats). Every 7 days, indicators of the excretory renal function (the level of diuresis and creatinine excretion) and activity of marker enzymes of renal tissue damage (lactate dehydrogenase and γ-glutamyl transferase) were determined in the urine of rats. After six weeks, we determined parameters of activity of the free radical oxidation process in the kidneys of rats posthumously and conducted the morphological study for formation of calcium deposits.
Conclusion: Experiments showed that in the control group there were characteristic signs of nephrolithiasis development: increase of LDH activity by 5.4 times, increase of free radical oxidation process activity in the kidneys, and formation of large concrements in the kidneys, the mean size of which was 298.8±34.2 μm2. In the test group, there was weakening of LDH and GGT activity and weakening of free radical oxidation process activity. There were no morphological signs of nephrolithiasis development in the kidneys. 

oxalate nephrolithiasis • Leu-Ile-Lys-His tetrapeptide • anti-lithogenic effect
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Received April 4, 2019.
Accepted June 7, 2019.
©2019 International Medical Research and Development Corporation.